Part:BBa_K3930006

Inducer Z3EV of the estradiol promoter Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal XbaI site found at 1021
  Illegal XbaI site found at 1324
  Illegal PstI site found at 666
  Illegal PstI site found at 837
  Illegal PstI site found at 1340
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 666
  Illegal PstI site found at 837
  Illegal PstI site found at 1340
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal XbaI site found at 1021
  Illegal XbaI site found at 1324
  Illegal PstI site found at 666
  Illegal PstI site found at 837
  Illegal PstI site found at 1340
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal XbaI site found at 1021
  Illegal XbaI site found at 1324
  Illegal PstI site found at 666
  Illegal PstI site found at 837
  Illegal PstI site found at 1340
  Illegal AgeI site found at 1527
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 1849
  Illegal BsaI.rc site found at 987
  

Introduction

The Z3eV assembly is composed of the promoter TEF1, the gene Z3eV coding for the activator Z3eV of the estradiol inducible promoter BBa_K3930007 and the transcription terminator of CYC1. The construction is based on the publication (Liu et al., 2020).

This sequence must be used with the promoter Z3eV (BBa_K3930007), for a gene regulated expression into S. cerevisiae.

Testing

This part is one of the component of the pFLEUR part (BBa_K3930000) and has been succesfully cloned into the LycoYeast S. cerevisiae strain genome. The Toulouse team could not test this part for reasons of time, and preferred to focus on the part characterizations that allow the production of α and β-ionone for the floral aspect of the violet perfume.

References

1. Liu J, Wang T, Jiang Y, Liu Z, Tian P, Wang F, Deng L. 2020. Harnessing β-estradiol inducible expression system to overproduce nervonic acid in Saccharomyces cerevisiae. Process Biochemistry. 92:37–42. doi:10.1016/j.procbio.2020.02.032.